188 BOTANICAL GAZETTE | SEPTEMBER 
gas produced by the growth of the germ in these solutions was 
analyzed by Mr. H.S. Bryan, of Purdue University, the results o 
which are given in Table III. The tests were made for CO,,0,), 
NH,, and CO, the difference being considered as hydrogea 
In the first analysis a much larger amount of nitrogen wa 
obtained than in any of the other cultures. The cultures for 
this analysis were several weeks old, and were perhaps not 
trustworthy. 
There were some irregularities in the amount of gas prv- 
duced, which cannot be accounted for. At one time 2 per cent. 
cane sugar bouillon containing no pepton when inoculated 
gave a large amount of gas, 20° being collected in each fer 
mentation tube; 2 per cent. cane sugar bouillon containing % 
pepton, inoculated at another time under exactly similar condi 
tions, gave only 2° of gas in each fermentation tube. 
The germ grown in bouillon to which had been added 2p 
cent. glucose at one time gave a very large amount of gas: # 
another time there was not enough gas produced to be analyze 7 
The gas produced by the germ as determined by analyses 
composed of a very small amount of oxygen, less ion Js . 
cent., carbon dioxide approximately 44 per cent., nitroget " ; 
per cent., and hydrogen approximately 30 per cent. bee 
tion was produced in sterilized beet juice, Pasteur’s solution, 
maple sap. No fermentation was produced by the 
the germ in bouillon to which no sugar had been added. 
SUMMARY. 
mination 0 
bacteria * 
ot broke! 
pe te 
It has been determined that a microscopical exa 
the tissues of diseased beets reveals the presence of 
the cells of the plant. The tissues of the plant are ® 
down, and the bacteria in all parts of the plant appeat “ ot 
same. Transfers of diseased tissue to the healthy rt ad ] 
resulted in changed appearances of the plant which 1 
almost certainly that the disease was transmitted. the plast 1 
The manner in which the germ finds entrance to to th 
has not been determined. The conditions most favore ‘ 
