228 BOTANICAL GAZETTE [ocToRER 
is about one hundred, so it is not probable that there has bees 
any multiplication since fertilization. Mitotic figures have never 
been seen in the oospore after that act, and it is probable that 
the oospore persists in this condition until the following spring. 
OILS AND OTHER FOODS. 
A thorough discussion of this subject would involve a much 
more elaborate microchemical study than was undertaken. It 
seems desirable, however, to record a few observations since 
these extra-protoplasmic substances are very conspicuous at cer 
tain periods of development and their presence often introduces 
serious difficulties of technique. No writer has given the sub- 
ject the attention that it deserves, and detailed study would 
doubtless yield valuable results. 
There are three substances which, when present, always 
appear as nearly spherical globules with the general appearance 
of oil, and they have undoubtedly been described as such by 
many previous observers. Further study may show that they 
are not true oils, and this seems quite probable, as they do ” : 
answer to all of the microchemical tests; but in the lack of 4 7 
precise knowledge of their chemical nature they may be ae 
sidered here under that name. All of them are found pe 
serial sections cut from paraffin, that is, in material which 4 
passed in bulk through the weak alcohols and rested in 7§ P* : 
cent. alcohol for weeks or months; which has passed i 
paraffin through chloroform and been cleared of out 
immersion for a few moments in xylol; and finally whic 
undergone the baths accessory to the stain employed. at 
The first oil is very abundant, existing in far greater deve 
than either of the others, and during more stages ae ee the 
opment of the fungus. It is found in the yous ee 
oosphere, and the periplasm. Diagrams showing “6 oa 
tion in the important periods of development are ae che age 
plates. fig. 46 presents it for an oogonium of ysis sonatio® 
shown in fig. 45. Fig. 63 illustrates its distribution @ we 
and it: should be noticed that the drops are ean = 
