: ie) THE COMPOUND OOSPHERE OF ALBUGO BLITI . 229 
meshes of the ooplasm are fine, but large in the periplasm 
where the meshes are more coarse. . Fig. 72 shows the condition 
ofan oogonium at a stage similar to fig. 70, a period some- 
what later than zonation. The distribution is much the same 
because the protoplasm has changed but little. fig. &7, a stage 
ter fertilization, indicates that as the meshes of the ooplasm 
become coarser the globules become larger; apparently some of 
the globules fuse to form the larger drops. A later stage 
similar to fig. 93 shows that the oil of the periplasm is nearly 
sthausted during the building up of the epispore. 
This most common oil is found in unstained sections as 
— Olackish or brown drops when the material has been killed by 
Flemming’s fixing fluid. It is usually absent from material fixed 
by chrom-acetic acid, or if present is in very small quantity. 
tthe material is allowed to remain several (six) days in the 
chrom-acetic fixing agent the oil appears in considerable quan- 
ity, very much as it does in Flemming’s material, but of lighter 
= olor instead of brown. When material fails to show the 
ae seems to be really absent, because several days’ immersion 
a cent. osmic acid solution fails to show its presence. 
eretore, it seems that the osmic acid of the Flemming’s fixing 
og the long immersion in chrom-acetic acid, so acts on 
st oll as to render it less easily soluble in the fluids that it 
: Sag fhe killing and the time when the sectians are 
: tiful a 7 oil when present takes a characteristic ane peau: 
he — from the safranin in the Flemming’s triple 
_Sombination. If it be ver dark from the action of the 
SMic acid, it is fre ‘ d er- 
“Mid to all quently necessary to employ hydrogen p 
May be le blackness, in order that nas best results 
ee i. from the stains. It is partially on account : 
at material killed by Flemming’s fluid is not so 
Sora) 
 €ttic i cytological study as that fixed by the chrom- 
- The Second oijl-lj 
'Y, possibly ¢; 
y ei 
* evident in oe 
ke substance is present only in very small 
ht or ten small globules in an oogonium. 
h Flemming and chrom-acetic material, is 
niet 
Fr 
