230 BOTANICAL GAZETTE [ocroner 
stained black by Heidenhain’s haematoxylin, but may be distin 
guished from the first oil by the fact that it does not take the saf- 
ranin when applied in the usual way. It is figured in the plates 
as black dots (figs. 51, 56, 59, 61, 64, 68, 69) ; in the antheridium 
in fig. 55; in the periplasm in fig. 80. No difference in stain 
reaction was observed between this oil-like substance and the — 
central globule of the coenocentrum, and they may be of similar 
nature, 
The third oil is only found in. the maturing oospore, first 
appearing while the secondary endospore wall is developing as 
globules of a clear honey-yellow color in the meshes of the proto- 
plasm ( fig. 95). It soon accumulates at the wall, and fig. 94 shows 
large drops in close contact with the forming secondary endo- 
spore, which has broken away from the primary endospore, prob- 
ably owing to the impact of the knife in cutting. The drops 
finally become larger and more numerous, as is shown in fig. 96. 
From a study of subsequent stages it seems probable that pi 
oil drops later break away from the wall irregularly, and unite nd 
form the several large drops that are often found in colder 
oospores. This results finally in the condition shown in fig. 9h 
where the entire central region is occupied by a curious irregular 
globular structure which stains much as the oils have stained in 
previous stages, but which is certainly not of fluid consistency: — 
It probably represents reserve food material. If this spre 
represents a genuine oil drop in the living spore, we may 
to do here with the shrunken and collapsed vesicle oF membrane 
that encased it in life, and whose contents have been “ 2 
the processes of technique. Its appearance would accor ee ” 
with this view. From its size and the time of devee a e 
the oospore it is surely the structure described as oil bx pT 3 
investigators, and is characteristic of the winter oospore: 
GENERAL CONSIDERATIONS. . 
eneral view of the : 
to previous ? 
= possible 
: It seems well before closing to take a g 
facts that have been presented, and of their relation 
knowledge; also to point out more clearly annie 
