19] THE COMPOUND OOSPHERE OF ALBUGO BLITI 235 
Other killing agents used were corrosive sublimate in satu- 
rated aqueous solution; hot corrosive-acetic-sublimate in alco- 
hol; Carnoy’s fluid; absolute alcohol; Hermann’s fluid; and 
Merkel’s fluid. Most of these gave far inferior results to that 
obtained by the chrom-acetic acid, and none surpassed it in 
effect 
Inorder to imbed in paraffin the material was transferred 
through 85, 95, and 100 per cent. alcohol to a mixture of absolute 
alcohol and chloroform, first of one third then of two thirds 
strength of chloroform. The specimens were left about two- 
hours ineach fluid, and were finally placed in pure chloroform. 
After the material had been left in chloroform for an hour a 
quantity of paraffin was added, and two hours later the material 
m= wamedon the bath. After two hours of gentle heat it was 
“moved to a warmer position, and later the most of the chloro- 
frm was poured off, melted paraffin substituted, and the whole 
j kept in the bath at a temperature of about 55°. It was found 
*antageous during the whole process of imbedding to use 
ey lion dishes, as by this means most or all of the chloro- 
aay off gradually by evaporation. Material was left 
twice ER 2 mont paraffin three or four hours, the paraffin being 
— Chlorof snged in the meantime to insure the removal of all 
~ a. It was then cast in a cake, the final paraffin hav- 
Bt melting Pvint of about 62°. Sections 3 to 5 win thickness 
Sig gil Jung sliding microtome and fastened to the slide 
t Mayer’s albumen fixative. 
aL Flemming: 
results. This stain demands the greatest atten- 
Peal use or failure is inevitable, as is well known by all 
8 a es In general, the best results were attained by a 
bs prolon a sa in safranin, followed by a rinsing more or 
ker of bien mM acid alcohol. The time here is entirely a mat- 
“20st usua a varying with the result desired ; 30-90 sec. was 
: the Slide After running down through the alcohols 
iia Placed in saturated solution of gentian-violet for 
5 min. Jt was then rinsed in water and placed in orange 
fon. 
