242 BOTANICAL GAZETIE [octonss 
PLATE XII, 
Magnification in all figures 1500 diameters. 
Fie. 42. A portion of mycelium, showing nuclei with prominent nucleoli, 
Fic. 43. Nuclei and cytoplasm flowing into a developing oogonium, nucle 
and vacuoles elongated and angular, nuclei too darkly stained to show 
structure. 
Fig. 44. The septum below the oogonium, a portion of mycelium and 
posterior end of odgonium, oogonial nuclei assuming the spirem stage 
mycelial nuclei still in resting condition with prominent nucleoli bet 
enlarged. 
Fic. 45. Oogonium and antheridium, nuclei of both in the spirem stage. 
Fig. 46. Shows location of oil drops in an oogonium of the condition pre 
sented in fig. g5. They were all drawn at one focus; a slight change of 
focus would have brought vastly more into view. 
Fic. 47. Showing adhesion of the oogonial Hautschicht to its wall in the 
neighborhood of the antheridium, nuclei overstained. 
Fic. 48. Adhesion as in fig. ¢7. Oogonial wall partly and irregularly 
corroded away on the side toward the oogonium. 
Figs. 49-55. Stages in the perforation of the wall preparatory t the 
entrance of the antheridial tube. : 
Fig. 49. Optical section of papilla, wall partly corroded away and bulging 
toward the antheridium. The dense protoplasm represented in black was 
stained a deep red by the safranin. 
FiG. 50. Sectional view of a condition slightly older than jig: #9: ox 
shaded portion of the separating wall took the stain differently from the 
and was apparently in the last stages of dissolution. 
Fic. 51. A papilla of different shape in a stage similar to the last. 
Fic. 52. Optical section, stain as in fig. 49. coe : 
F1G. 53. A papilla becoming bubble-like, walls very thin, slight'Y°™" 
as though due to imperfect killing, watery vacuoles apparent, their © 
staining homogeneously with the sia ee airections, 
Fig. 54. Bubble-like papilla expanding irregularly in all di 
tents highly vacuolate, wall extremely thin. igi ng in eithet 
Fig. 55. Wall almost perforated, but showing no marked bulg! 
direction, 0, oogonial, a, antheridial side. punded 
Figs. 56, 57. Young antheridial tubes with a cellulose wall and s 
by a dense sheath of the protoplasm of the ng ae voachiol 
Fic. 58. Protoplasm beginning to collect in masses, ee fixed 
metaphase, some shown’ in polar view (preparation from mat ae 
Flemming’s agent and stained by haematoxylin). 
