340 BOTANICAL GAZETTE [ NOVEMBER 
angle of the V in front, as one might expect if the spindle fibers 
are assumed to be exerting a pull upon them. They are always 
more slender than the parent chromosomes, which would be the 
case if a division of the original substance had taken place. 
Occasionally a V will straighten out and lie along the spindle 
fiber, reaching almost from the equator to the pole. 
The spindle in Convallaria is formed in the same way as in 
Lilium. The disappearance of the membrane is exactly coin- 
cident with the appearance of the kinoplasmic threads which 
immediately penetrate the nuclear cavity, and also extend 
outward into the cytoplasm (fig. 72). Very little light, how- 
ever, could be thrown on the fate of the nucleolus, and its dis- 
appearance was very sudden. The multipolar spindle is not 
very distinct in this plant (fig. 73), and the poles scarcely ever 
extend much beyond the limits of the old nuclear membrane, 
and are often difficult to distinguish at all. The bipolar spindle 
is usually truncate at the poles, but unlike that of Potamogeton, 
it is broad and barrel-shaped, possibly due to the large number 
of chromosomes (jig. 74). In this respect it is similar to 
Lilium. In no case was there even so much as a granule present 
at the pole, or any thing that could be mistaken for a centro- 
sphere. A strong nuclear plate follows the division, resulting 
in a cross wall separating the cell into two hemispherical parts. 
SECOND NUCLEAR DIVISION OF THE MOTHER-CELL. 
The resting stage between the first and second divisions 9 
Convallaria is very short. One may often find the nuclei at 
one end of an anther in the cell-plate stage, while those at bas 
other end have formed the nuclear plate for the second han? 
The daughter nuclei do not seem to pass entirely into 4 nowt 
resting condition. So far as could be determined, no saan 
ever appears, nor does the nuclear membrane become well et 
oped, however a very delicate membrane may often be amr 
The chromosomes apparently retain their identity fete 
this stage. From the pole view it can be determined that t* 
still possess their V-shaped form (fig. 16). 
