308 BOTANICAL GAZETTE [OCTOBER 
vigorous, and hence a thicker surface growth resulted. Numerous 
small colonies also developed on surface. : 
Gelatin stab—Growth takes place along the stab and also on 
the surface of the media. The growth along the puncture was fili- 
form; on the surface it was thin, spreading from the point of punc- 
ture, the margin undulatory to lobate-lobular. No liquefaction. 
Gelatin plate colonies—These appeared in two to three days. 
The deep colonies much the same as those in agar Petri plates. Sur- 
face colonies more spreading, forming a very thin growth with irregular 
undulatory margins, some darker in color than surface colonies. 
Surface colonies under low power showed the center to be denser 
than the margin and finely reticulated. Toward the margin the 
reticulations are much coarser than in the center. 
Potato.—Growth was vigorous and characterized by always being 
straw color. The growth was quite markedly raised above the sur- 
face and soon covered the entire plug. 
Bouillon.—In meat bouillon growth appeared after two days as 
a fine granular substance that remained in suspension, The culture 
is at first slightly acid to litmus, but becomes alkaline after two weeks. 
Glucose bouillon.—Growth the same as in the meat boullion, with 
the same reactions. The change from acid to alkaline reaction 1S 
much slower than in the other media. 
 Saccharose bouillon —Growth at first more vigorous than in either 
the bouillon or glucose bouillon; acid at first, neutral after seven 
days, and alkaline in two weeks. 
Lactose bouillon —Showed the same general characteristics as Sac 
charose, acid at first, then neutral and alkaline after two weeks. 
Litmus milk—Showed no change until ten days, when there was 
a distinct alkaline reaction. No coagulation ever occurred. After 
fifteen days the medium was quite blue, with a slight whitish precip 
tate at the bottom of the tube, This was not granular, but sub- 
gelatinous, and when shaken into solution settled again to the bottom. 
In a tube two months old the liquid becomes very blue and alkaline. 
Milk.—Showed no change except a slight yellowing in color. 
Scarcely any experimental work was done in growing the Bacillus 
at different temperatures. SAVASTANO (4) states the optimum tem- 
perature for the olive knot to be between 32-38° C, and SMITH (9) 
