1906] YAMANOUCHI—POLYSIPHONIA VIOLACEA 413 
pericentral cell later also gives rise to a group of auxiliary cells. The 
carpogonial branch consists of four cells which are formed successively 
as shown in diagram 2, D, E. It is somewhat bent, so that the ter- 
minal cell lies almost above the pericentral cell. This terminal cell 
becomes the carpogonium and develops the trichogyne. The mitoses 
concerned with the development of the carpogonial branch are illus- 
trated in figs. 94-99, and invariably showed 20 chromosomes at 
metaphase. As the result of these successive mitoses, the carpogonial 
branch extends at one side of the central axial cell, with the pericentral 
cell situated between them (diagram 2, E). 
The nucleus in the fourth or terminal cell of the carpogonial 
branch divides (fig. roo) to form two nuclei (fig. roz), each with 20 
chromosomes, one of which becomes the female gamete nucleus, 
while the other enters the trichogyne that is formed at once. The 
upper end of the cell pushes out as a delicate process which contains 
almost from the beginning one of the two nuclei, the other remain- 
ing in the basal swollen region of the cell called the carpogonium 
(figs. 102, 10 3, 104), which corresponds to an oogonium. The forma- 
tion of the trichogyne completes the development of the female organ, 
Whose parts in longitudinal section are shown in jig. 103. 
The trichogyne.—The trichogyne nucleus, as a rule, is situated in 
the middle region of the trichogyne, which has about the same breadth 
throughout its tubular cavity, but becomes constricted below where 
it joins the carpogonium. No plastids could be found in the trichogyne. 
The presence of a trichogyne nucleus in the red algae has been a 
subject of some controversy. Scumitz (69) described a large single 
or several small granular bodies, which stained like chromatin, in 
the trichogyne of Batrachos permum monilijorme and Gloeosiphonia 
before fertilization, but he gives no interpretation further than the 
few words, “Derivate des Zellkerns der weiblichen Zellen?” Eight 
years later Davis (17) observed in the same species of Batracho- 
‘permum an unmistakable nucleus-in the trichogyne, staining with 
haematoxylin as a dark blue body. OLTMANNS (55) also observed 
the granule within the trichogyne of Gloeosiphonia, but he regarded it 
as having no connection with the nucleus. ScHMrpLE (68) failed 
to find the nucleus in the trichogyne of Batrachospermum, and 
OstERHOUT (57) contends that it is not present. Wotre (86) 
