468 BOTANICAL GAZETTE [DECEMBER 
among other forms of the Ascobolaceae, in most of which the spores 
are projected for some distance through the terminal pore of the ascus. 
Modifications of the usual strengths of Flemming’s chromacetic 
osmium fixing fluids were exclusively used in the preparation of the 
material. Portions of the substratum upon which apothecia grew 
were removed and dropped into the fluids. Beside the mature fruits 
many younger stages were thus obtained. The sections were stained 
with safranin gentian-violet orange-G combinations and also with 
Heidenhain’s iron-alum haematoxylin. The sections were cut 5-10 # 
thick. 
HARPER (’95, ’96, :05) has shown that the ascogonia of the mil- 
dews consists of a much curved row of cells, each of which contains 
a single nucleus, with the exception of the penultimate cell, which 
contains two or several nuclei, and which forms the ascus in Sphaer- 
otheca, out of which grow the ascogenous hyphae in other forms. 
BARKER (:03, :04) also observed the same kind of ascogonium 
in Ryparobius; while CLaussEN (:05) finds several such ascogonia 
in Boudiera. The youngest ascogonium which I have thus far 
observed in Thecotheus consisted of a row of several cells which were 
already multinucleate (jig. 1). HARPER (’96) figures and describes 
perforations in the cross walls in the ascogonium of Ascobolus con- 
necting the adjacent cells: One of the cells of the row is larger. 
It is from this particular cell that the ascogenous hyphae arise, and 
out of which the nuclei enter the ascogenous hyphae from it and 
the neighboring cells. As soon as the ascogonia become empty 
they undergo disintegration. BARKER (:04) finds each cell of the 
row in Ryparobius at first uninucleate and then binucleate or occa- 
sionally quadrinucleate. From the cells of the row the ascoge ous 
hyphae delevop. He does not distinctly say that they develop from 
any particular cell of the ascogonium. CLAUssEN (:05) finds that 
the gametes of Boudiera contain several nuclei, which however fuse 
in pairs in the oogonium. Several walls are formed, so that the 
resulting ascogonium consists of a row of several cells from which 
the ascogenous hyphae arise. Thecotheus agrees with Ryparobius 
and Boudiera in that the ascogenous hyphae do not arise from any 
single cell of the ascogonium, but from any or all of them (jigs. 1 and 
2). The youngest ascogonia which I have been able to find were 
