172 Dr. H. Charlton Bastian on the 
the generation within the resting-spores of Vaucherta of inde- 
pendent forms of life of a very low order, resembling Am@bee 
or the simplest forms of Actinophrys, but forms of life which. 
are so heavily freighted with indigestible matter as to give 
them but a poor chance of undergoing further development. 
March 20, 1903.—During the last three or four days I 
have again examined many specimens taken from the second 
batch of developed Vaucheria resting-spores contained in the 
open vessel, These examinations have been made after a 
long interval, and during this time much of the water had 
evaporated. Though rather more than ten months had 
elapsed from the date when the resting-spores were gathered, 
many of them were found to be still undeveloped. They 
were mixed with much débris from dead - filaments, with 
empty cases of resting-spores, and with a large quantity of 
pale green Vaucheria filaments emanating from resting-spores 
which had germinated. Some of these filaments were still in 
continuity with, though others were separated from, the spores 
from which they had issued. 
Each of the resting-spores in connexion with living fila- 
ments contained one or more of the pigmented Ameeboid 
spheres. ‘These were now found to be almost motionless, and 
none of them had wandered out into the green filaments. 
They were therefore probably some of the resting-spores that 
had recently developed, after long confinement under un- 
favourable conditions, with the result that the pigment 
Amebe were less active than those which had been produced 
at an earlier period. 
On the other hand, many of the spores and filaments were 
dead, and from them all, or almost all, the chlorophyll-cor- 
puscles had disappeared, though these filaments contained 
one, two, or more of the spherical Am@be, and many of them 
were in a more fully developed condition than any I had 
previously seen. The specimens had lapsed into a resting- 
stage and were perfectly motionless; but they were seen to 
possess a wider and more distinct border of protoplasm, stained 
of a slightly brownish colour, but free from pigment-granules, 
Some of these specimens, as in Pl. XIV. fig. 4, A (x 875), 
showed clear indications of a commencing segmentation of 
this peripheral protoplasm, while in others, as in B (x 375), — 
segmentation had actually occurred into a number of minute 
monads, whose movements had to be arrested with a dilute 
solution of osmic acid before the photograph could be taken. 
Two or three of these monads (rather out of focus) may be 
