396 Dr. H. Charlton Bastian on the Origin 
nation. I accordingly made a number of subcultures, but in 
no instance have I succeeded in obtaining a growth ” *. 
I had by this time only two or three of the apples left, so 
I placed them in the incubator at a temperature of 76° F. and 
there left them for eight days. On section two of them were 
found to present the brown discoloration in the usual situa- 
tions to a well-marked extent. Some portions of the brown 
tissue were broken up with needles, placed in a dilute solution 
of the “‘ mastzellen”’ stain, and were afterwards submitted to 
careful examination with the microscope. There was certainly 
a very distinct increase of the cocci-like bodies in the primor- 
dial utricle, remaining unstained, as in Pl. XXVI. fig. 8, C 
(x 700), though all the other granules in the cells had become 
strongly stained. In some places the cocci were seen in 
distinct rows, branching and crossing one another, as in 
fig. 8, J (x 375) in the neighbourhood of the letter, so that 
they looked like spores within minute filaments fT. 
A further careful and prolonged examination revealed the 
fact that very many of the cells showed, in whole or in part, 
on or in the lining membrane, the cocci-like bodies as in C, 
though in other cells there were none of them. There was 
often a tendency for these bodies to arrange themselves in 
rows (as in EK and J), and in places to grow into delicate 
filaments (asin D and F). Such filaments were also seen 
occasionally crossing the cavity of the cell, and having spore- 
like bodies at intervals. A few larger filaments or hyphe, 
such as G, were likewise seen, together with toruloid corpuscles, 
asin H. ‘The spore-like body in G, from which the hypha 
has developed, is only a little larger than one of the cocci- 
like bodies to be seen near the lower left corner of C. Any 
doubts as to the reality of these latter bodies being embryo 
Bacteria may be set at rest by comparing them with what is 
shown in P]. XXV. figs. 2 and 4. All the organisms found 
here, as with these shown in the figures above mentioned 
from other vegetable cells, were similar in refusing to stain 
with all ordinary dyes. 
Although the first examinations of these apples showed, 
therefore, only very doubtful organisms or none at all, a 
* Some time previously Dr. Nabarro, of University College, had been 
similarly unsuccessful in obtaining growths from a potato which had been 
treated in the manner I have detailed on p. 390, and in which organisms 
seemed to be present in an early stage. Such lack of success after trials 
with a few culture media is, of course, far from disproving the presence 
of microorganisms. 
T Unfortunately this particular photograph was taken at a low magni- 
fication, but C and each of the others were taken at 700 diameters. 
