54 BOTANICAL GAZETTE [JULY 
spores had germinated, a number of them were carefully marked, and 
on the 21st, when the colonies had become visible to the unaided eye, 
six of them were carefully transferred to tubes of acid bean stems and 
three to tubes which had not been made acid. October 25 a similar dilu- 
tion culture was made, in which on the following day a number of colo- 
nies were carefully marked. These plates 
were then allowed to stand until November 
14 in order that any foreign growths which 
might be present should have an opportunity 
to develop, when eight of the marked colo- 
nies which still remained entirely distinct 
from all other growths were transferred to 
acid bean stems. 
The first growth to appear on bean stems 
is invariably the production of a few scat- 
tered, comparatively thick, more or less branched, stromal growths, 
which arise above the substratum at the point of infection. These 
shortly become covered with a growth of flocculent white mycelium, 
and from this center the entire surface of the stem and of the liquid 
becomes covered with a dense gelatinous looking stroma, which on 
the surface of the stem is covered with the mycelium (fig. 4). 
In the course of ten days or two weeks there is usually an abundant — 
production of sori, which are of an olive-green color when seen by — 
transmitted light, but give to the surface of the stroma a glistening — 
lack appearance when viewed by reflected light. In the older cultures _ 
the stroma has invariably become sa 
deep salmon color, the cause of which 
has as yet not been determined. We 
FIG. 9. 
Fic. 10. 
spherical, olive-green, perithecium-like bodies, covered with a scam 
mycelial growth, which possibly presage the development of an ascige 
ous form. In no instance, however, have asci been found in these bodie® 
To determine whether the fungus studied is the cause of 
disease under consideration, on October 30 thirty-six inoculations were 
