1900] BRIEFER ARTICLES 55 
made on sections of apple limbs. Twelve sections, each from 0.75 to 
1.50 inches in diameter and 4 to 6 inches long, were selected and 
divided into groups of four sections each. One of these groups was 
thoroughly washed in a solution of mercuric chlorid (1 to 1000), after 
which it was thoroughly rinsed in water. The other two groups were 
left untreated. The sections in each group were numbered 1, 2, 3,4, and 
upon each section three inoculations were made. On sections 1 and 3 
in'each group the inoculations were made by scraping up the epidermis 
with a flamed scalpel and applying directly to the exposed cortex and 
cambium small portions of bean stem culture bearing an abundant 
mycelial growth and conidia. In inoculating sections 2 and 4 care 
was taken to select portions on which the bark was uninjured and to 
make the applications without in the slightest abrading the epidermis. 
The sections when prepared were placed in fruit jars containing 
Moist sand, which had been thoroughly sterilized by steam heat. In 
about a week after the inoculations were made slightly discolored areas 
were observed about several of the points of infection, and by November 
20, three weeks from the time they were made, these areas had devel- 
Oped all the characteristics of the disease as seen in nature; being 
