84 BOTANICAL GAZETTE [aucust 
since the kinoplasmic portions of the cell in division were nearly 
always well fixed in it. It proved in this respect to be muth 
superior to the weaker solution for the tissues studied. The 
material was kept in the killing fluid 30-48 hours, washed in 
running water, hardened by carrying through the different grades 
of alcohol and imbedded in paraffin in the usual way. Some | 
care was necessary to avoid plasmolysis in the early stages of 
hardening. I found that the objects could not be left in the 
lower grades of alcohol for a very long time; 30 minutes in I§ 
per cent., 45 minutes in 30 per cent., and one hour in 50 per 
cent. seemed to be about the maximum limits. Above 50 pet 
cent. more latitude as to time could be allowed. 
into the gentian-violet solution 20-25 minutes. They Wee © 
again rinsed in water and passed rapidly through a weak orange 
solution (1 part saturated solution orange G in H,O + 1 ae , 
H,0), dehydrated, clarified quickly in clove oil, and mounted # 
balsam. For Staining objects killed in the fluids containili 
mercuric chlorid, Zimmermann’s fuchsin iodine green® . 
Heidenhain’s haematoxylin, preceded by Bordeaux red. We 
ground stain, were found most serviceable. 
# Morph, u. Phys. d. pfl, Zelikernes 6, 
