98 BOTANICAL GAZETTE [AUGUST 
The similarity in staining of this substance to that of the 
cell walls, together with its presence in the region of the spindle 
in which the cell wall appears later, I have taken to signify the 
presence of a carbohydrate substance destined for the formation 
of the new cell wall. Whether it is at all analogous to the 
previously described layer around the spindle in the cytoplasm 
of the larch, I am not able to say. The fact may be of some 
significance in this connection, that in a late stage of division i 
the larch a similar orange layer appears in the cytoplasm arount 
each daughter nucleus. Possibly it shows the presence of 
material destined for the formation of the walls of the pollen 
grains. This fact may be correlated with the fact that the © 
division of the pollen mother cell often does not follow the fist ~ 
nuclear division. But in the stages showing the young perma 
nent cell plate, such a substance was generally invisible, thougi 
in some cells a slight indication of it was seen. There woul 
seem to be in these cells very slight or no aggregation of reserve 
cell-wall material. It is interesting to note that in the dividing 
pollen grains of Iris and Hemerocallis no orange stained inter 
filar substance was seen. Here, of course, there is no cell wal 
formed between the two cells. 
The relation of the spindle fibers to this orange substance i 
worthy of notice. As above mentioned, they retain their chat j | 
acteristic color, but they often appear greatly attenuated in bes 
region. It would seem that the substance had crowded thea F 
into such a condition (figs. 28, 29), and that it retarded tht 
previously described processes of thickening and separating © 
the fibers. | 
oospheres of Fucus. In the Saprolegniaceze Trow has sugs® 
that the so-called cellulin granules are a form of reserve cellul / 
which may be used to close the opening made by the breaking. 4 
ofahypha. I have seen preparations of Saprolegnia in whit 
the young cell wall cutting off the sporange stains strongly ¥ 
