1900 | CHLOROCYSTIS COHNII 107 
for all the other observers of this plant have spoken of a color- 
less neck which projects beyond the surface of the cell and 
through which the zoospores make their way. Wright, however, 
has said to Miss Whitting (16) that when he found Chlorocystis 
Cohnii developed in the interior of tissue, the cells were some- 
times quite globular. Certainly the figures of Lagerheim (12) 
and Reinhardt (14) do not correspond to the description of a 
plant possessing such a protuberance. If the Chlorochytrium 
inclusum of Kjellmann (Q) is finally to find a place within the 
genus Chlorocystis, as has been suggested, we have still another 
example of a form without the neck-like protuberance. After 
the examination of dried specimens of Chlorochytrium inclusum 
Kjell. I am not inclined to think that it is a Chlorocystis ; although 
the published figures are strongly suggestive of that genus. It 
is certain that at no time, among the hundreds of specimens of 
Chlorocystis which were examined, was there anything that 
resembled a colorless protuberance. It may be that the varying 
habitat has something to do with the difference in aspect which 
this alga often presents; at any rate it seems probable that the 
presence or absence of a colorless tubular portion through which 
the zoospores may escape is not of much importance. 
hen the zoospores are liberated they swim about for a 
length of time varying from a few minutes to two hours. No 
difference was discernible in the rate or length of activity of the 
two kinds of spores. In almost every case the spores escaped 
perfectly free and independently of each other, but in a very few 
instances it appeared as though they might have been enclosed 
a delicate membrane as in Chlorochytrium. If there was such 
ag it must have been very frail and was suggested 
ofa es the arrangement of the zoospores than by any actual 
nat ee It always seemed to break up before any reagent 
a. € added to demonstrate it, and it is quite possible that 
bie = of the kind exists. Such a membrane enclosing the 
cae "es could not be of any significance from a systematic 
a Point, for even in forms where it occurs frequently, there 
Conditions which bring about its total disappearance. 
