1900] CHLOROCYSTIS COHNIT 109 
colorless neck is pushed out, and this penetrates the Entero- 
morpha frond between its cells and pushes them apart. When 
an entrance has been gained in this manner, the neck widens 
until the whole cell appears funnel-shaped, and this, after further 
growth, assumes its mature spherical condition. Ina few instances 
zoospores were observed which had germinated without having 
come in contact with the host-plant, and it is an interesting 
fact that some of these sent out colorless tubes of a considerable 
length (fig. 8). These were all found in cultures of various kinds 
and may have been due to some unknown abnormal condition. 
In the first published account of this plant, the zoospores 
were described as escaping from the mother cell without pos- 
sessing any color. These colorless zoospores developed into 
colorless plants which remained so until they had nearly reached 
adult size when the protoplasm commenced to develop ‘green 
cromules.” ‘These cromules,” says Wright (16), ‘‘arise as 
minute points along the inner surface of the cell wall from 
whence they radiate to the nucleus giving the appearance of a 
number of necklaces hung in loops.” Although I looked care- 
fully for some such condition in my material I was unable to 
observe anything abnormal or unusual. The green zoospores 
gradually developed into mature green plants with definite 
chromatophores as described. 
Resting spores were observed in material that had been kept 
in the laboratory for some time and had been allowed to dry 
"P partially. They are formed by the thickening of the wall of 
tie mature plant and the contents rolling itself into a solid mass 
of irregular outline, The spore thus becomes of a darker green 
shade, and the pyrenoid is lost in the increased density of the 
ceil contents. 
Be ei - seen from the foregoing that a number of points 
rae es to the structure and development of Chore 
cea, a have been considered by former investigators as 
ra deiaey can no longer remain as such. The habit of the 
si api and it certainly cannot be regarded as a uni- 
ophyte. The chromatophore is quite as apt to line 
