1900] MULTIPOLAR SPINDLE IN GLADIOLUS 151 
cones first approach each other and finally unite in two groups 
in the form of a bipolar spindle, as shown in fig. zo. 
From the above observations, and from previous work on 
other forms, it would seem that there are several types of spindle 
development in the higher plants. It would be imprudent to 
classify all of these probable types until the early stages of 
many of the forms have been more thoroughly investigated. -At 
present we have at least three forms which have been thoroughly 
worked out and which differ from one another sufficiently to 
warrant us in distinguishing them as types. These are repre- 
sented by Equisetum, Cobaea, and Gladiolus. 
SUMMARY. 
The above observations may be summarized as follows: 
As nuclear division approaches, a granular zone accumulates 
about the nucleus. This zone in every respect resembles the 
perikaryoplasm so characteristic of the pollen mother cells of 
obaea. 
A close network or felted zone of kinoplasmic fibers is 
formed immediately outside of and completely surrounding the 
nuclear wall. This js probably developed from the perikaryoplasm. 
This network grows out into several projections which 
become the cones of the multipolar figure. 
The nuclear membrane persists until the cones are almost 
fully developed. 
The spindle fibers are formed by the elongation of the 
Meshes of the network composing the cones. 
: Neither the nuclear wall, nucleolus, nor linin take any essen- 
tial part in the formation of the achromatic figure. 
The Cones of the multipolar figure fuse and arrange them- 
Selves in two Stoups and form a bipolar spindle. 
THE Universiry OF CALIFORNIA. 
1894 LITERATURE. | 
3 BELAJErr, W. Zur Kenntniss der Karyokinese bei den Pflanzen. 
seg Bone a 
AVIS,B.M. The spore mother cell of Anthoceros. Bor. GAZ. 28:89. 
