1900] MULTIPOLAR SPINDLE IN GLADIOLUS 153 
EXPLANATION OF PLATE XII. 
Figures drawn with Abbe’s camera lucida, Zeiss homog, immersion objec- 
tive one twelfth, apert. 1.25, compensating ocular no. 6. 
Fic. 1. A pollen mother cell showing the cytoplasm differentiated into 
three zones; the outer cytoplasm stains a light gray-blue ; the perikaryoplasm 
stains a light orange, and the weft or felted zone of kinoplasmic fibers stains 
blue; the nuclear wall is int dth ] contains a large nucleolus and 
several curved chromosomes, with a small amount of linin threads. 
Fig. 2. The weft of kinoplasmic threads is commencing to push out into 
several projections, preparatory to forming the cones of the multipolar figure ; 
the network of the weft appears more distinctly. 
Fic. 3. A still later stage in the development of the projection, with 
distinct cones formed. 
Fic. 4. A still later stage showing the elongation of the meshes and 
the formation of fibers by the pulling out of the network. 
Fic. 5. A slightly older stage than that of fig. 4. 
Fic. 6. A later stage when the cones are nearly fully developed; the 
Outer portions of the cones consist now of distinct fibers, and it is only at the 
base of the cones that the network of the original weft can be distinguished ; 
the nuclear wall has as yet shown no sign of breaking down, and the 
nucleolus still persists. 
1G. 7. The first indication of the nuclear wall breaking down, The -_ 
Mosomes now come in contact with the base of the cones. 
Fig. 8. Shows the same as fig. 7, but a little more advanced. 
Fig. 9. The nuclear wall and the nucleolus have disappeared ; several ed 
the cones have fused, and the chromosomes are attached to the fibers at the 
base of the cones. 
FIG. 10. The mature spindle, with the chromosomes at the equator and 
ut to move to the poles. 
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