232 BOTANICAL GAZETTE [OCTOBER 
to the distribution of the nuclei. Later the furrows proceed in 
every case so as to cut off uninucleated masses approximately 
equal in size. This later period of cleavage is characterized in 
many cases by the aggregation and rounding up of the denser 
cytoplasm about the nuclei so as to leave hyaline regions midway 
between each pair of nuclei, thus predetermining in each case 
the plane of cleavage to be followed later by the cleavage fur- 
row. This type of cleavage results immediately in every case 
in the formation of uninucleated cells whose nuclei, however, 
may still be in a state of division. In the end, the entire pro- 
toplasm of the aethalium has been cut into uninucleated seg- 
ments which are at first naked bits of protoplasm. Later each 
cell becomes surrounded by a wall and constitutes a spore. 
Turning now to the phenomena of nuclear division, we may 
note first of all that the structure of the resting nucleus com 
forms, in spite of its small size, to that of the nuclei of other 
fungi and the higher plants. Nuclear membrane, chromatin 
(nuclein), and nucleole are present, and are differentiated by 
staining with safranin, gentian-violet and orange, just as sha rply 
as they are in the pollen mother cells of the lily. The nucleole 
frequently lies in a clear space (fig. 70), a& is so frequently seen 
in the nuclei of the root-tip of the onion: 
The nuclei, however, are too minute for the successful study 
of the prophases in spindle formation. In the equatorial plate 
stage (figs. 3, 5, 11) the spindle is sharply ‘differentiated. 
shows rather sharp-pointed poles which may be more peat 
stained at their tips. Broad-poled spindles, such as those figure 
by Strasburger for Trichia, are not found in Fuligo. The ea 
somes stain deeply in the equatorial plate stage. In polar ued 
it is possible to count the number with considerable speaginte? 
The great number of these figures to be found in “— 
Fuligo at this stage make the material especially favors 
such study. The chromosomes are relatively short and 
and form a very regular equatorial plate, all of them bie om 
tically in a single plane, so that in polar views they are pra a 
cally all in focus at once. From a study of a large nanan Er 
