A NEW CHROMOGENIC MICROCOCCUS 265 
moist layer, If the agar is older the edges are likely to be 
irregular or lobulated. 
The reaction of the agar, ranging 15 acid, neutral, or 15 
alkaline, Fuller’s scale, produces little perceptible effect, although 
the early development may be slightly more rapid on neutral 
At 37° the growth is very limited, and there is no pigment, 
but growth proceeds normally on removal from the incubator. 
Light seemed to have little influence upon pigment production ; 
cultures standing on a shelf near a north window where no sun- 
light entered had about the same appearance as those kept con- 
‘inwally ina dark locker. Development on glycerine and 2 per 
sent. glucose agar showed no variations from ordinary growth. 
siato—The micrococcus refused to develop on either 
= or alkaline potato at room temperature or in the incu- 
or, 
Milk —Cultures at 20° C, showed no change beyond a deposit 
ida thick surface ring of the same characteristic color as the 
aie gelatine cultures. Lactose litmus milk brought out 
_ oir very well, the litmus itself turning a more vivid blue, 
aay an alkaline reaction. In course of a month or more, 
squid showed traces of peptonization without coagulation. 
ea n.— Became cloudy in forty-eight hours and remained 
k Ee atge beyond the slow accumulation of a salmon- 
ane No production of indol nor reduction of nitrate. 
in the bulb ig ube.—Dextrose 2 per cent. showed slow greet 
in the clog and accumulation of pink sediment. No cloudiness — 
‘ ed arm and no gas. 
rally win, Tada morphology, etc.—The micrococcus aati 
ounted eidinary stains and is decolorized by Grae s aeae ; 
a ations reveal small cocci measuring about 0. é 
Satcina, gi Re ag irregularly with no particular indication 
Mens in har, ®- or strepto-coccus form. The unstained speci- 
Vibration iy drop showed no motility other than the ae 
Uistaineg . st measure less than 1 #, somewhat smaller than 
Pecimens of M. agilis. No spores observed. 
