266 BOTANICAL GAZETTE [ocToBER 
Upon cultivation in gelatine hanging drop under +, immer- 
sion lens, the cell multiplication appeared as shown in fig. g. | 
was unable with this power to see the actual process of division, 
but there was little doubt that it took place in only two planes 
giving rise to a typical staphylococcus grouping. That division 
did not occur in three directions was evident 
g 8 B from cultures in liquid gelatine or broth, in 
g $3 a which the cells divided regularly into groups 
of two cells each, which remained together a 
short time, then separated into free single 
cocci. 'The best series observed was that 
bre figured in the drawing, the development occur 
ring in a solid gelatine drop as follows: twenty-four hours after 
sowing, in which time multiplication is well begun, a group of 
three cells was brought into focus, carefully examined, and drawa 
at 11:30 A.M. I examined the group at intervals of a few minutes 
without seeing any signs of division, but at 2: 30 it was evident that 
two cells had taken the place of one in the group. At 4:00P™ 
there were five cells, and two hours later (6:00 P. M.) an oblong 
group of six cells could be seen in one place. At 8:00 the next 
morning ten cells were clearly visible, and the group had begun 
to acquire the circular form characteristic of all the niet 
advanced colonies in gelatine culture, although in agar hanging 
drop, as on an agar plate, the colony form remains irregular. 
2:30 P. M. of the second day of observation, only a slight ne 
had taken place, fourteen cells being in focus in one plane, ge = 
ing a rosette-like figure. Afterward the growth was very pp 
the last drawing of the colony having been made five days @ 
sowing. All colonies showed the same glomerulus structure 
_ In the endeavor to identify this micrococcus with the a 
varieties already described, I found some unexpected difficu is 
First, the liquefaction of gelatine by this form is at pore 
pass almost without remark, making it doubtful 10 ee “ - 
Migula’s two classes the germ should be placed.’ It _ witd 
sary, therefore, to examine his whole series, especially 
>MIGULA: System der Bakterien 2: —. 1900. 
