1900] CURRENT LITERATURE 283 
the cause of “club root” in cabbages and turnips. Woronin, who studied 
this form more than twenty years ago, found that it does not form sporangia, 
but that the spore masses lie free in the cells of the host. Eycleshymer, in 
Jour. Mycol. 7: 79-88. 1892, gives a clear account of its life history and 
distribution in the United States. The present paper is concerned with the 
more minute details, Infected roots were cut into very small pieces and 
were treated with Flemming’s stronger solution for twenty-four hours. The 
author thinks that the advantages of a more prolonged treatment are entirely 
imaginary. Sections were cut 2-3 # in thickness, and were fastened to the 
slide with distilled water without any further fixative. The Flemming triple 
Stain gave the best results. Dilute Delafield’s haematoxylin, followed by 
eosin in clove oil, and also the gentian-violet method according to Gram, 
save good results. The peculiar method of nuclear division in the vegeta- 
le amoebae is worthy of special mention. In the resting condition this 
mucleus has a membrane, a nucleolus, and an extremely delicate chromatin 
network, As division begins, clearly differentiated chromatin granules appear 
inthe place of the network, the granules having no genetic connection with 
the nucleolus. A plate evidently derived from the chromatin granules is then 
we hear the nucleolus. At this stage there is a sort of one-sided “ achro- 
matic figure” with its base resting upon the chromatin plate and its apex at 
the nuclear membrane, but the figure afterward acquires a symmetrical 
“spect, the drawings in the plate bearing considerable resemblance to bipolar 
tg The nucleolus then divides transversely, and the two resulting 
. hia Positions on opposite sides of the chromatin plate, Bydeoee » now 
Panetta disk with chromatin granules imbedded in its Pee 
ie plate then splits, and the two parts, each accompanied by a 
» Move toward the poles of the spindle, and two daughter nuclei are 
_ This method of division is of greater interest because the nuclear 
type, in the plasmodium is simultaneous and of the usual bipolar mitotic 
§ a author's summary of the entire paper is about as follows 
— of infected cells arises by the repeated division of a primary 
division 
ea : : : S 
a the growth of the infected cells numerous multinucleate 
3. fa *ppear, which multiply without fusing to form a plasmodium. 
ir lag Condition the amoebae of Plasmodiophora are remarkable for 
4. Sagat and especially for their unique mode of nuclear division. 
in oth © mode of nutrition of the amoebae seems to be different from that 
she aeomycetes. 
Place in og coos fuse into a plasmodium, characteristic changes take 
‘4 The § Structure of the body and in the nuclei. oe 
*xhausted, ormation of a plasmodium takes place only after the host cell 1S 
