324 BOTANICAL GAZETTE [NOVEMBER 
Histology —The anatomical study of Lessonia littoralss, here- : 
with presented, is based upon a series of slides made for meat 4 
my request by Mr. Harold L. Lyon, Instructor in the Botanical — 
Department of the University of Minnesota. The material was q 
all passed through the alcohols and embedded in paraffin inthe 
usual way. The sections were cut upon a Minot precision q 
microtome, and transferred gradually from clearing agents back — 
to formalose water. Many of them were stained with aniline 4 
water-safranin and aniline-blue, and permanent mounts in forma- 4 
lose water were prepared by sealing the cover slips at the edges. 
Mr. Lyon found that the ordinary mounting media distorted 
the sections, and after a few trials of other media he settled 7 
upon the formalose water as the best. I shall consider the 3 
different areas of the plant in order, beginning at the proximal 4 
regions. 7 
The holdfast.—As has already been stated, the holdfast are 4 
of our specimen was much distorted and confused by the mex 4 
tricable matting together and coalescence of originally separate - 
branches. The primitive disk area was not definitely . 4 
tinguished. Sections through the central and basal rene ® 4 
the holdfast showed a most confusing and irregular tissue, ae 
dently the result of appression of hapteres to each others 
surfaces. In cavities of the irregular mass of the hol a, 
unattached hapteric branches were found, and these sho 
ordinary dichotomous branching, cylindrial shape, and smo" 
green surface characteristic of the higher Laminariacee. 
did not essentially differ in appearance from t 
ocystis, described by me last year, nor from t 
previously described by Foslie and others. BS 
than 2™ in diameter in ultimate branches, 
edly within narrow vertical limits. Cross sections § 
to be composed of a pretty homogeneous t . 
chymatous tissue in which the cells are of varying SiZ@>s ae 
- ic : surface the diametet 
ing from 6-30 in diameter. Towards the suri io 
of the cells lying in the radius of the cylinder 1s : 
there is a general superficial cambial area extending af | 
