366 BOTANICAL GAZETTE | DECEMBER 
the expense of the outer cytoplasm, begin to collect in greater 
abundance at opposite sides of the nucleus and to be pushed up 
into dome shaped prominences. This condition is very well 
shown in fig. 3. The threads of granules run nearly tangential 
to the nucleus, extending out some distance at the side, and it 
is obvious even at this time that the axis and the bipolarity 
of the spindle are already determined. It is rarely the case, 
however, that both poles are equally prominent from the begin- 
ning; commonly one develops considerably in advance of the 
other. A curious relation between the position of the chromatin 
in synapsis and the first formed pole of the spindle was 
observed so frequently that it cannot be regarded as acci- 
dental. In elongated cells in which the nucleus is situated 
excentrically, the first pole of the spindle is formed on that 
side of the nucleus where there is the greatest amount of cyto- 
plasm, while in synapsis the chromatin was almost invariably 
observed to be gathered within the nucleus to the side farthest 
from the center of the cell; that is, the chromatin in synapsis 
and the kinoplasm are gathered towards opposite ends of the cell. 
Such a relation between chromatin and kinoplasm may be true of 
all the mother cells, but it can be traced only in those cells which 
have the distinction of a long and a short axis, since the polar- 
ity of the spindle is not apparent until some time subsequent to 
synapsis. These peculiarities will be referred to again when the 
second division is considered. Every care was taken to avoid 
the illusion into which an observer might be misled by the effects 
of irregularity of infiltration of the fixing or other reagents, and 
the conclusion was reached that these relations have a real exist 
ence and significance in the living cell. 
The writer inadvertently had an excellent opportunity - 
examine a condition of the cell somewhat resembling SY ae, a 
Finding that the young sporangia, which are ensheathed ¥ 7 
close covering of hairs, did not sink readily in the killing ee 
he first moistened some of them for a moment with om? 
They sank immediately, but afte: wards turned out to be ight 
unfit for study. It was perfectly easy to trace the path of 
