1900] ACHROMATIC SPINDLE OF OSMUNDA 375 
Such a relation between the fibrillae of the kinoplasm and 
the cytoplasmic reticulum as Blackman (§) reports in Pinus, 
and Lawson (8) in Codaea scandens, could not be verified. 
Between well developed spindle and cytoplasm are the three 
stages, (1) dotted fibers, (2) granules, (3) amorphous kinoplasm 
(structure too delicate for the microscope to reveal). The same 
phases in reverse order were traced in the first formation of the 
spindle. 
This investigation was conducted in the Hull Botanical 
laboratory of the University of Chicago during the spring and 
summer of 1899. The writer, while assuming full responsibility 
for the views expressed, takes pleasure in acknowledging his 
indebtedness to the members of the Botanical Staff for their 
courtesy and encouragement, and especially to Dr. Bradley M. 
Davis, under whose more immediate direction the work was 
undertaken, 
+" Since the foregoing account was written (in February 1900), a com- 
pehensive work on karyokinetic problems (15a) has been issued by Stras- 
burger, in which, among other topics, he discusses the divisions of the spore 
other cells of Osmunda, and the general formation of achromatic spindles 
“plants. He distinguishes two types of spindles, those possessing ened 
“mes and those without such controlling centers. The latter, which are 
‘racteristic of higher plants, are again subdivided into multipolar polyarch 
‘pindles, such as those of the spore mother cells of Equisetum, and multi- 
tar diarch spindles, such as are common in various spermatophyte root- 
"8S; and he see 
§ the same origin and reaction to stains, 
iz and mantle fibers, but supporting and attracting fibers pi 
oo). He pronounces against a power of movement in the af 
“toth oo Selves, and attributes their withdrawal from the equatorial 2 . 
: = _ of the attraction fibers. This conclusion is quite eee ne 
“is use t expressed in the preceding pages. To the view that the ae 
'P to help complete the achromatic spindle, my observations, 
t 
— Not “ : too 
“ontradictory, are not altogether favorable; the spindle nee 
