IIo BOTANICAL GAZETTE 
The experiments thus far show that the maximum amount of 
water in the plant will not induce the basal primordia to develop. 
The opposite method was then tried, i. e., supplying the other por 
sible factors in regeneration and at the same time withdrawing water 
from the plant. 
Experiment 18.—Eight plants were used, the soil allowed to dry 
until the leaves wilted, and the stems then cut off a few centimeters _ 
above the ground. No more water was added and the soil became 
quite dry. The remaining part of the stems wilted and the cotyledons _ 
began to shrivel. In spite of this the primordia developed on six of 
the plants;. on the other two they started but soon withered com — 
pletely. The six that developed grew very slowly and apparently — 
. suffered severely from lack of water. Three of them were ‘then 2 
watered, and at once recovered turgidity and grew normally. On 
the other three the young shoots reached an average length of 3% — 
and then died. Here the buds started to develop with a turgidity 
much below the normal, and continued slowly against a decreasing — 
turgescence until the death point was reached. This part of the : 
experiment was repeated on several occasions and always with the 
same result. Check plants under the same conditions, but with the 
stems not removed, showed no development of the buds. The fo 
lowing more accurate method was then used. | 
Experiment 19.—To the weak nutrient salt solution in which - 
plants were frequently grown was added KNO, to make up solutions 
of the following gram-equivalent proportions: 3, 3, 4, 4 % a 
%> 19: To each of these were transferred plants whose roots 1° 
been grown in the usual nutrient salt solution.. In four days | 
‘Toots in the 3, 4, and 4 gram-equivalent solutions were ent 
plasmolyzed and killed, and the stems and leaves wilted. 10 
¢ solution the roots were partially plasmolyzed, though not killed, 
and the leaves were just flaccid. In the + solution the roots Se ’ 
quite healthy, and also the shoots, but growth was very slow. ™ 
the other solutions the plants were vigorous and grew rap! 
Another set of these solutions was made up, and in each were 
plants with vigorous roots that had been developed in nutrient 
solutions, and each stem was cut off. As before, those in the 4 
and 4 solutions were killed by. plasmolysis; in the } solution 
