174 BOTANICAL GAZETTE [sePreuen 
division of the chromatin precedes each of the two mitoses, the 
second fission occurring during the meta- or anaphase of the first, 
or heterotypic division. 
In my first study of Podophyllum, in 1896, certain steps in the 
development of the heterotypic chromosomes were never clearly 
understood, and at that time the phenomena in question were attrib: 
uted to poor fixation. On the appearance of STRASBURGER’S papet 
on Galtonia candicans, I again took up the study of Podophyllum, 
because of the facts just stated, and because the reduced number of 
chromosomes in this plant is only eight. This study has resulted in 
a clearer understanding of the phenomena in question, and for com- 
parison my study is being extended to other plants, among them 
being Lilium candidum. Although my study is not yet completed, 
and owing to the delay in publication which may ensue, it has been 
thought best to make public a brief statement of the conclusions 
reached in reference to the heterotypic chromosomes in the pollen 
mother cells of Podophyllum peltatum. 
It may be stated at the outset that my earlier description of the 
resting nucleus in the pollen mother cell of this plant is substantially 
correct. The fine linin network contains many small ¢ eee 
granules of uniform size and distribution. One or more nucleoli may 
phenomenon is normal. Following synapsis, the loosening 
contracted ball results in the chromatin spirem; and as soon as 
spirem has emerged from the balled-up condition, oF shortly after 
wards, and has assumed a more regular arrangeme 
seen to be split longitudinally. I am not, at this writing, Preps" 
to state definitely whether the spirem is formed double as © agi 
by Bercus and ALLEN, but in the contracted condition portions 
the threadwhich occasionally extend out free from t 
loop could sometimes be seen to be double, or appe@ 
lengthwise. After all this is not strange, when We b 
the spirem is derived from a network, and nothing is more P 
thread paral 
nt, it 16 Cee 
the mass 35? — 
remember that : 
than that in the transformation of the net into the 1 
threads of consecutive meshes would be approximated. It 
