246 BOTANICAL GAZETTE [ocToBER 
Experiment 41.—The buds at all the nodes were removed from 
a larger number of plants, leaving the growing tip and the basal 
primordia; the latter did not develop. When the tip also was removed 
they started promptly, even though they were separated from it bya 
distance of 7o°"—six to nine internodes. Figure 1 shows one with 
three internodes. 
It has been shown that no 
amount of wounding short of the 
complete severance of the stem 
will produce this result. GOEBEL 
(2, p. 386) says that in Circaea if 
the central orthotropous shoot be 
allowed to grow in a dark chamber, 
it has the same effect on the lateral 
plagiotropic shoots as if it were 
removed; that is, one or more of 
these become orthotropous. 
Experiment 42.—Young plants 
of Phaseolus were taken when the 
epicotyl was 5 or 6°™ long, the first 
Sper ta cence pa Sakai of leaves jt Sl 
soon developed shoots. the apex with the leaves directed 
into a dark chamber. The lower 
part of the stems were in light and the roots in soil. The parts ™ 
the dark elongated rapidly and soon were completely etiolated, but 
in no case did the buds below develop. Similar experiments with 
Salix showed this would not cause the axial buds below the part 
the dark to develop. It would look as though a complete removal 
of the apex is necessary to start into activity the latent growing pom's 
below, but experiment 43 shows that this is not so, for there the pe 
of the four young plants were passed into a bell jar and sealed alr 
tight with wax, and through the bell jar a continuous current of 
hydrogen gas was passed. The growth slowed down, and after 
about twenty-four hours ceased entirely; and in a few days the but 
in the axils of the cotyledons below started to develop and grew quite 
vigorously, Upon the removal of the bell jar the apices of the shoots 
continued to grow. This shows that only a cessation of certain 
