310 BOTANICAL GAZETTE [ocToBER 
For examination it is then mounted in Canada balsam, the preparation 
being permanent for years, as is also the case with the unmounted material. 
Corn starch may also be stained by the use of this method, but in the 
staining of potato and maranta starches it is necessary to use weaker iodin 
solutions. I have not evolved entirely satisfactory methods, however, for 
uniformly staining these latter starches. 
It may be of interest to state that the foregoing method has certain 
features which are similar to those of the gentian-violet method used in 
demonstrating the so-called continuity of protoplasm in the vegetable cell 
wall (15). In the study of the continuity of protoplasm a swelling reagent, 
such as sulfuric acid, is used and a.comparatively short time is consumed 
in the whole operation; whereas in the method proposed for the staining 
of wheat starch the water used may be considered a swelling agent acting 
on the grain during a longer time. The analogy in the results are 0 
striking that students who are interested in the study of the continuity of 
protoplasm will do well to compare their results on the cell wall with those 
obtained in the study of the wheat starch grain by the method just described. 
The author acknowledges his indebtedness to Miss FLORENCE YAPLE 
for valuable assistance in the preparation of this paper.—HENRY KRAEMER, 
Philadelphia College of Pharmacy. 
LITERATURE CITED. 
I. KRAEMER: Bot. GAZETTE 34:341. 1902. 
2. NAGEL, C.: Die Starkekérner. 1858. 
3. Raspatt: Citation by von Mout in Bot. Zeit. 1'7:226. 1859. 
4. FritscHe: Poggendorff’s Annalen der Physik und Chemie 32:—: 
5. ScHImMPEr: Bot. Zeit. 38:881. 1880; 39:185, 201, 217- 1881. 
6. Meyer, A.: Untersuchungen iiber die Starkekérner. Jena. 1895. 
7. Myttus: Ber. Deutsch. Chem. Gesells. 20:688. 1887- 
8. MEINEKE: Chem. Zeit. 18:157. 1894. 
9. Stocks: Chem. News 57:183. 1888. 
0. Sryrert: Zeitschr. Angewand. Chem. 1:15. 1886. 
11. Rouvrer: Compt. Rend. 114:749. 1892. 
12. Kisrer: Liebig’s Annalen der Chem. 283:360. 1894- 
13. RovuvreR: Compt. Rend. 120:1179. 1895. 
14. NAcELI und SCHWENDENER: Das Mikroskop. Leipzig. 1877: 
15. Kraemer: Proc. Amer. Phil. Soc. 41:174- 1902- 
1834- 
