1900] DEVELOPMENT OF THE POLLEN GRAIN 89 
centrosomes, although the cytoplasmic granules may appear in 
this region as well as in all other portions. 
Although irregular in outline, in the daughter nuclei the 
chromosomes remain more or less distinct in Symplocarpus, and 
connected to one another by linin threads (fig. 9); while in Pel- 
tandra the. chromosomes are more closely united into a dispirem 
ribbon, and not readily differentiated (fig. 21). In neither case 
is there any reappearance of the nucleolus before the second divi- 
sion is entered upon, this being in marked contrast with what 
obtains in certain dicotyledons under study ‘at the same time— 
especially Bignonia. 
In the telophase of division in Peltandra, the kinoplasmic 
fibers largely disappear from the middle portion of the cell after 
the formation of the cell plate has begun, and these fibers are 
then found to be abundantly concentrated at the periphery. 
They pass around in the plane of the long axis of the nucleus, 
entirely surrounding the latter. They form a complete weft 
around the periphery of the daughter cells, especially adundant 
in the equatorial region (fig. 27). When the development. of 
the cell plate is complete, and the two daughter cells are distinct, 
these radiations are separated at the extreme edge; and from 
that point they radiate as before (fig. 22). They are evidently 
concerned in the formation of an independent wall, even in the 
two-cell stage. 
THE SECOND DIVISION. 
After the first division in Symplocarpus, some peculiar con- 
dition renders staining a very difficult process. The safranin- 
gentian-orange gives a diffuse pink in the general cytoplasm, 
and the chromosomes appear indefinite in outline. Iron hae- 
matoxylin often works to better effect if the sections are deeply 
stained and much decolorized. In this stage it is necessary to 
make the sections very thin. The difficulty in staining persists 
until the microspores become free, and then again the material is 
ae The second division has not been studied in Pel- 
andra, 
