264 BOTANICAL GAZETTE [ APRIL 
daughter cell bulges more and more strongly towards the latter, 
and finally the discharge of the spore is accomplished, as in 
other instances where a papilla for dehiscence is formed, slowly at 
first and then more rapidly ; while during its discharge, the basal 
septum reverses its position, the pressure exerted by the contents 
of the adjacent cell pushing it back into the monosporangium as 
the latter empties (figs. 2-4), the process continuing after the 
spore has escaped until the cavity is wholly obliterated, so that 
in a few hours the position of the sporangium is merely indicated 
by the slightly projecting margins of the orifice through which 
the spore escaped. ; 
The spore, which is discharged with a force only sufficient to 
carry it a very short distance from the filament, is quite spherical, 
completely filled with chloroplasts, except for a small area 
(jig. 5) which is free from them, and possesses not evena slight 
amoeboid motion. In the Van Tieghem cell a few of the spores 
became disorganized in a short time, while a great majority, 
secreting a distinct wall, began to germinate on the day follow- 
ing their discharge; and the young plants thus formed continued 
to grow, forming branching filaments, for more than a week, 
when the culture had to be abandoned. 
This type of reproduction, which was frequently met with in 
the material examined at Cocoanut Grove as well as at Daytona, 
was very uniform in all cases, but just as I was preparing to 
leave the former locality, a tuft of small plants was found grow- 
ing on submerged grass culms in water more than usually subject 
to tidal influence, among which forms having the normal type of 
sporulation were associated with others in which it was some- 
what different. Although the peculiar characteristics of this 
second type of sporulation may be the result of unfavorable 
conditions and without special significance, it may be convenient 
on account of their small size to distinguish the spores formed 
in connection with it as microaplanospores. é 
Formation of microaplanospores.—The formation of the cells 
destined to produce microaplanospores resembles the first steps 
in the formation of the macroaplanosporangia in that any cells 
