Re ee a ee eee Se ake eee 
1900] CULTURES OF UREDINEZ IN 1899 269 
adhered. The spores were scraped from the host material with 
a knife blade and placed on definite areas of the plant to be 
infected, the parts marked by bits of string, and a bell jar placed 
over the whole plant. After a day or two the bell jar was 
removed. With most species, if the operation had been success- 
ful, the results began to appear in six to fourteen days. 
In order that the simple procedure here outlined may uni- 
formly give information that can be relied upon, many small 
precautions must be observed that cannot now be spoken of. In 
every case it is wise to first make a drop culture, to be sure that 
the spores are viable. 
Inthe following outline of the work accomplished it is impos- 
sible to go into much detail, or mention all the items of interest 
that are naturally brought to mind. It is also impossible to 
adjust the nomenclature in accordance with the new knowledge 
which the cultures have furnished. 
1.. Puccinta Puracmitis (Schum.) Korn. 
The host of this species, Phragmites communis, does not grow 
in the vicinity of Lafayette, Indiana, where the work was done, 
and I am indebted to Dr. J. J. Davis of Racine, Wis., and 
Professor C. E, Bessey, of Lincoln, Neb., for teleutosporic 
material. Abundance of zcidia were obtained by sowing the 
teleutospores upon Rumex crispus and R. obtusifolius, thus con- 
firming the results obtained by European investigators. The 
dates are as follows: 
May 2, Teleutospores (Wis.) sown on Rumex crispus ; May 12 spermogonia ; 
ay 15, ecidia. 
May 4, Teleutospores (Wis.) sown on Rumex obtusifolius ; May 15, spermo- 
gonia; May 16, ecidia. 
May 9, Teleutospores (Neb.) sown on Rumex crispus May 15, spermogonia, 
May —, ecidia 
May 9, Teleutospores (Neb.) sown on Rumex obtusifolius ; May 15, speTmo- 
gonia; May —, ecidia. 
The preliminary drop cultures showed the most vigorous 
germination of teleutospores obtained with any species, and far 
Surpassing most of those under observation. The sowings also 
