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1900 | CULTURES OF UREDINEZ IN 1899 275 
11. PuCCINIA PERIDERMIOSPORA (£. & 7.) Arth. 
In studying the grass rusts it was noticed that a form on 
Spartina, well represented in American herbaria and usually 
called Puccinia Phragmitis, had uredospores similar to those of 
Puccinia Vilfe, just described. I searched through my collec- 
tion of Aicidia to see if any species with corresponding apically 
thickened spores could be found, and was rewarded in the case 
of 4cidium Fraxini Schw. With this morphological hint as the 
- sole guide cultures were undertaken. 
Spartina does not grow within many miles of Lafayette, and 
I am indebted to the kindness of Professor C. E. Bessey, of Lin- 
coln, Neb., and Mr. H. H. Hume, of Ames, Iowa, for teleuto- 
sporic material upon Spartina cynosuroides. The teleutospores 
germinated with marked vigor, and were first sown on Ptelea 
trifoliata, the small plants obtained being mistaken for Fraxi- 
nus. When the mistake was discovered, and sowings were made 
on Fraxinus viridis, infections were first secured. As no small 
plants of Fraxinus were available, the spores were sown upon 
cut twigs placed in water in the greenhouse, and upon a low 
branch of a large tree out of doors. There could be no fear of 
spontaneous infection, as the species does not occur in the 
region, either upon Spartina or Fraxinus, but the usual precau- 
tions were taken. The sowings were both successful. The dates 
are as follows: 
May 8, Teleutospores (Neb.) sown on P/e/ea trifoliata ; no infection. 
May 9, Teleutospores {Neb.) sown on Ptelea trifoliata ; no infection. 
May 9, Teleutospores (lowa) sown on P/edea trifoliata ; no infection. 
May Is, Teleutospores (Neb.) sown on P/elea trifoliata ; no infection. 
May 17, Teleutospores (lowa) sown on cut twigs of Fraxinus viridis; May 
24, Spermogonia; June 6, excidia. 
May 17, Teleutospores (Neb.) sown on tree out of doors of Fraxinus viridis ; 
May 20, spermogonia; June 8, zcidia. 
SUMMARY. 
The following are the eleven species of U 
ecidial and teleutosporic forms have been definitely connected 
by cultures: 
redinez, whose 
