1900 | | FERTILIZATION OF ALBUGO CANDIDA 311 
EXPLANATION OF PLATE XXII. 
The material, fixed in chrom-acetic acid, was cut 5 # thick and stained on 
the slide with safranin, gentian-violet, and orange G 
All figures sketched with an Abbé camera under the Zeiss apochromatic 
objective 2™™ ap. 1.30 in combination with compensation oculars. 
Figures 1-8 magnified 1000 diameters; 9-12, 2250 diameters. 
Fig. 1. Oogonium showing the first trace of the accumulation of the pro- 
toplasm in the central region preparatory to the differentiation of the ooplasm ; 
nuclei entering or in stages of mitosis 
Fic. 2. Oogonium at the stage af zonation ; nuclei mostly in metaphase 
and near the inner boundary of the periplasm; coenocentrum very large and 
surrounded by a zone of lightly staining cytoplasm; antheridial tube pene- 
trating the periplasm 
1G. 3. Oosphere differentiated with large deeply stained coenocentrum 
and smaller female nucleus ; antheridial tube in the interior of the oo plasm 
and about ready to discharge the sperm nucleus; another nucleus in the 
neck of the tube, near the antheridium. 
FG. 4. Oosphere heute’: antheridial tube (a) in adjacent section ; 
coenocentrum and female nucleus shown 6). 
1G. 5. Antheridium discharging its contents into the oosphere; sperm in 
amass of dense cytoplasm; coenocentrum very deeply stained, with female 
nucleus in close proximit 
Fic. 6. Sexual nuclei fusing in oospore; coenocentrum less conspicuous 
than in earlier stages. 
Fig. 7. Sexual nuclei approaching one another; coenocentrum frag- 
menting. 
Fig. 8. Fusion nucleus; coenocentrum dissolved into an ill-defined 
region of dense cytoplasm. 
FiG. 9. Resting nucleus with prominent nucleolus and delicate linin net- 
work, 
FiG. 10, Nucleus in metaphase of mitosis ; spindle intranuclear. 
Fic. 11, Nucleus in anaphase of mitosis: nuclear membrane still persist- 
ing, ‘ 
Fig, 12, Daughter nuclei after mitosis; old nuclear membrane entirely 
dissolved. 
