1900 | DEVELOPMENT OF TRICHURUS AND STYSANUS 319 
stock tubes in the laboratory. It is needless to say that through- 
out the cultures the differences between the two forms remained 
constant. The difference in the size of the two plants alone 
would be sufficient to remove all doubt as to their specific dis- 
tinctness. In the course of the cultures, however, so many 
points of resemblance between the two plants were found, 
including a small form of fructification new to Stysanus, that it 
seems worth while to mention them here. 
The spores of Stysanus stemonites are in general oblong or 
lemon-shaped, but, as in Trichurus, they are more or less irreg- 
ular, and extremely variable in shape and size (fig. zr). They 
are usually more pointed than in Trichurus. Under the micro- 
scope the spores are dilutely olive-brown, almost hyaline, having 
the usual dark refractive line around the border. They measure 
from 6-7y in length by 3-4 in diameter, but vary outside of 
these limits. 
Germination of the spores begins immediately after sowing. 
The process takes place exactly as was described for Trichurus, 
that is by the formation of a thick primary germ tube (fig. 72). 
After eighteen to nineteen hours the mycelium has reached the 
stage of development shown in fig. 14. The tubes are rather 
slender, rarely exceeding 3-4 in diameter. The protoplasm is 
somewhat indistinctly granular and vacuolate. 
The colonies formed by the mycelium are smaller than those 
of Trichurus of the same age. The hyphae do not show a great 
tendency to spread out in the agar but remain within a small 
compass. At the end of four days growth the colonies are 
scarcely more than 3-4™™ in diameter. The mycelium branches 
abundantly and irregularly, forming a dense mat whose center 
is raised slightly above the agar. The center is even more 
stroma-like than in Trichurus. The colonies soon become cov- 
ered with a gray gonidial growth which extends almost to their 
margin. This growth consists of Penicillium-like gonidiophores 
resembling those described for Trichurus. In this case, however 
they are much more abundant, thus differing in a marked man- 
ner from Trichurus, in which they are never sufficiently numerous 
