OBSERVATIONS OX FILARIA VOLVULLS 



305 



Soon the embryos take on a more definite shape, lying curleci up 

 in the cell with clear amnionic fluid separating them from a very 

 distinct lining- mambrane. On staining with logwood the curled up 

 embryos show minute nuclei. The cells vary in size and average 

 32 [x by 34 [X. 



Followingthe utérus up, someof the (.'uried embryos are seen lu 

 hâve ruptured the cell membrane and to be lying free in the cavity. 

 They gradually uncurl, and 

 fînally are to be seen packed y ^ I 



in rows and interlaced with 

 one another. Development 

 does not proceed at the sa me 

 rate in both branches of the 

 utérus, as in one branch 

 curled embryos can be seen, 

 with the other branch full of 

 straight embryos Iving along- 

 side. The embryos hère mea- 

 sure 180 to 200 [i. in length by 

 4 [x in breadth. Eventually 

 both branches of the utérus 

 are seen to be filled with 

 straight embryos. 



The branches of the utérus 

 eontaining embi-yos can be 

 traced distincly to within 

 lmm5 of the head. After this 

 owing to the clearness of the 

 spécimen they were more 

 diffîcult to follow, and I was 

 unable to détermine the vagina or its ojjening. 



The alimentary opens terminally and at the sides two indistinct 

 canals can be made out. 



Embryos. — The free embryos fourni lying in tlie cyst measure 

 250 a in length by 3 a to 6 [x in breadth. The head is rounded. Tlie 

 tail is very sharp with a taper of about I/o of the length of the 

 body. A collection of Une granules is seen running along the body, 



; X 



Fig.4. — Fragment of the body of the female, 

 showing the narrow intestine and two 

 large utérine tubes, with embryos at dif- 

 férent stages of their developmi-nt. 



