664. REPORT—1896. 
II. The action of sea-water upon the growth of the Z. typhosus. 
IIT. The bacteria present in the alimentary canal of the oyster. 
IV. The infection of the oyster with the B. typhosus and its removal 
by washing. 
V. The green coloration and green disease in oysters. 
I.—The Identification and Differentiation of the Bacillus typhosus and 
B. coli communis. 
We have systematically tested the majority of the chief differential 
reactions upon samples of Bacillus typhosus and B. coli obtained from 
numerous sources, and have in all cases found unmistakable differences 
between the two bacilli. 
Table showing Differences of Reaction. 
3 Fermentation. Indol Cosgula- | Potassium Iodide 
ounce Glucose Gelatine} Reaction tion Potato Gelatine 
A. B. typhosus. 
1. Institut Pasteur 3 none none none very small growth 
2. From spleen of ty- 
phoid patient +; ” ” ” 
32 Prot. Delépine . . ” ” ” ” 
4. Prof. Wright (Net- | A slight trace | clot slow- | a5 
ley). f | ‘ly formed 
5. (| ” none | none} Nt 
6. | Dr.Sims Woodhead , | a A = | ie 
ie ” ” ” | ” 
e: \ Dr. Kanthack {| 2 2 ay | z 
“ot Rate Yj ” i wet a v” 
10. Institute of Preven- | 
tive Medicine eal “ s rs fi 
B. B. coli. 
1. Institut Pasteur . | well marked marked marked | growth abundant 
 spinks. «| 
2. Prof. Delépine . Sy) “A slight pink f Bes 
3. Prof. Wright . dl af | _ pink FP _ 
4. | | Slight pink | + 9 
5. | 5 marked pink) ts ns 
6. | ” ! pink | ” ”” 
4 ;Dr. Sims Woodhead. | " Liaise nelde soal " 
9. \ ” | ‘ } ” i 
10. | 7 ” 9 ” 
i 3 | ” ” ” 
12. i = f bc slight pink , A 5 
13. | Dr. Kanthack 1 ‘ Sokent ; / i 
14. Institute of Preven- | / 
tive Medicine ; | + * | F F 
Summary of Constancy or Variability of Reactions. 
A. For B. typhosus :—1. Fermentation test. Constant (Burri and 
Stutzer have shown gas formation). 2. Indol reaction. Slight indication 
in one case. 3. Milk coagulation. Slight clot in one case. 4. Potassic 
todide potato gelatine. Characteristic invariably ; very little use as a 
separating medium. 5. Potatoes. Constant with usual precautions. 
6. Reaction in gelatine. Marked differences of rate of diffusion. 
