216 
the individuals of the three pairs in which the union was plainly seen to 
occur. However the water and food vacuoles were seen to be carried 
across intact especially in the pair shown in fig. 2, in which case each 
Amoeba contained two large water (?) vacuoles. The resulting individual 
contained the four. 
Regarding the phenomena following the union of the two individuals 
I can give only enough data to show that there are in all probability 
resulting phenomena of interest. Immediately after copulation in each 
case, the resulting Amoeba remained comparatively quiet for a period 
of several minutes and then, in the case of the last two pairs of indivi- 
duals observed (fig. 2 and 3), the resulting Amoebae crawled into a 
mass of debris and were lost. The individual Fig. 3. 
resulting from the first pair observed to copulate 
(fig. 1) was followed for a-period of four hours. 
This copula after remaining quiet for a few mi- 
Fig. 2. 
Da 
nutes, having the shape shown in e, then began sending out pseudo- 
podia as in f and crawled into a mass of bacteria where it remained for 
about ten minutes assuming shapes similar to g and apparently feeding. 
It then took the shape shown in h, and moved rapidly across the field in 
a comparatively straight line and toward a line of bacteria near the 
edge of the slide. There were many other Amoebae on the slide and 
almost all had also taken the shape shown in k, and were moving in the 
same direction as the one under observation. In the posterior and 
pointed end of the copula as many as three vacuoles would appear and 
would then burst into one which would then contract. The “club 
shape” of the Amoeba is the typical Amoeba limax shape and the fact 
that it was the predominant shape of all the individuals observed, is 
one reason for believing this to have been Amoeba limax. 
