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As it came nearer to the line of bacteria the copula rounded up (2) 
and crawled slowly among the bacteria. This was about a half hour 
after copulation and the Amoeba stayed here during the remainder of 
the time that it was under observation, as did the other Amoebae 
which had been moving toward the bacteria. 
About an hour after copulation I noticed a large clear space formed 
in the copula, which had much the appearance of a large vacuole as 
shown inj. The Amoeba now became more or less “knotted” in appear- 
ance and the wall of the clear space which had been taken for a vacuole 
became thicker and brownish in color, with a small semi-clear space 
near the center (k). Two hours after copulation and an hour after its 
first appearance, this structure had become very distinct and seemed 
near to the outside of the body and at one time appeared to be almost 
Fig. 4. Fig. 5. 
cut off. During the remaining two hours during which the Amoeba was 
observed it remained in much the shape shown in / and finally crawled 
out of sight and in amongst some other Amoebae and was lost. Many 
other Amoebae on the slide showed this same “brown body” and other 
similar bodies were seen lying loose upon the slide, but this was the 
only case in which what appeared to be the development of one of them 
was observed. 
During the period between the arrival of the zygote Amoeba, above 
described at the line of bacteria and the end of the observation, the 
Amoeba approached or was approached by one near-by individual five 
separate times, but in each instance, after touching, the two separated. 
These may have been merely collisions. Similar collisions of individuals 
in a condition prepared for copulation might lead to fusion. This indi- 
vidual, being itself a zygote, perhaps repulsed instead of attracting the 
others which it touched. 
My work was unavoidably interrupted for a day and not being 
able to find any more of the Amoebae copulating after that, I mounted 
some of the material. Among those successfully stained with Delafield’s 
Haemotoxylin and mounted, only two individuals show more than one 
