417 
the fact now published by MM. Regnard and Blanchard amount 
simply to a confirmation of an observation published thirteen years ago. 
Further than this MM. Regnard and Blanchard have given 
an erroneous account of the history of the discovery of haemoglobin in 
the lower animals and of our present knowledge of its distribution. 
The first spectroscopic observations on the haemoglobin of the Earth- 
worm were published by me in 1867 (Journal of Anat. and Physiology) 
and in the same year Nawrocki published his researches on the sub- 
ject. Rollett did not demonstrate haemoglobin in the insect-larva 
Chironomus, for he did not make use of the spectroscope. 
The spectroscopic evidence was furnished by me in 1869 when 
I also published the fact of the existence of haemoglobin in Planorbis, 
Cheirocephalus and Daphnia. In Vol. IV of Pflüger’s Arch. f. 
Physiologie 1871, I published the fact of the existence of haemoglobin 
(ascertained spectroscopically) in the muscular tissue of the buccal 
mass of the Gastropod Molluses Limnaeus and Paludina — and in the 
vascular fluid of the Chaetopods Eunice, Cirrhatulus, Nereis, Terebella, 
Tubifex, Limnodrilus, Lumbriculus and Nais, and of the leeches Nephe- 
lis and Hirudo. 
In the Proc. Roy. Soc. 1873. No. 140. I added to this list the 
red corpuscles of Chaetopods G/ycera and Capitella and of the Gephy- 
rean Phoronis: the vascular fluid of some Nemertines: the red blood- 
corpuscles of the Lamellibranch Solen Jegumen: the nervous tissue 
of the ventral ganglionated cord of Aphrodite: the muscular tissue of 
the buccal mass of Littorina, Patella, Chiton, Aplysia and of the giz- 
zard of Aplysia, and of certain muscles in different groups of Verte- 
brata. 
Since this I have published three additional instances of red-co- 
loured corpuscles impregnated with haemoglobin viz. in the blood of 
the Lamellibranch Arca (See English edition of Gegenbaur’s 
Comparative Anatomy) and in the coelomic fluid of the Gephyreans 
Thalassema Neptuni, and Hamingia arctica K. and D. (this Anzeiger 
1881. No. 87 and Ann. and Mag. Nat. Hist. Jan. 1883). 
Other instances of the occurrence of haemoglobin have been pu- 
blished by Van Beneden (1873) who detected it in a special vas- 
cular system in Lernanthropus and Clavella, by Hubrecht (1875) 
who found it in the nervous tissue of Nemertine worms and in corpu- 
scles contained in the vascular fluid of some species and in the pro- 
boscidean sheath of others and lastly by Foettinger (1880) who 
found it in corpuscles in the body cavity of an Ophiurid Echinoderm. 
The above list is sufficient to shew how entirely MM. Regnard 
and Blanchard have failed to make themselves acquainted with the 
