110 BULLETIN OF THE 
in confinement in glass aquaria, which condition must be given its proper 
significance, and ‘may have deterred them from clinging to the parents. 
In this connection let me mention an observation which has been 
repeatedly observed. in dredging our common “ basket-fish.” The egg 
and early development of this animal (Gorgonocephalus Agassiz’) is 
unknown, but the young with arms with a single bifurcation have 
repeatedly been brought up from the bottom clinging to the disk of an 
old adult. It is very necessary to find out whether or not the basket- 
fish is viviparous, as this would possibly indicate. 
The young Amphiure are born at intervals, in a continuous series, 
not all at the same time. There are well-developed young of different 
sizes in the same parent. It was not possible to tell from the size, in all 
cases, Whether the mother or adult had young in the body. Swollen 
specimens were ordinarily gravid, but many large specimens were without 
young. 
When the disk of many swollen specimens was looked at from above 
(abactinal region), one or more of the interradial regions was observed to 
have a reddish color and to be more swollen than the remaining. On 
dissecting these specimens to learn the cause of the color and apparent 
abnormal condition, they were found to harbor in their bodies bundles 
of claret-colored ova. The development of these ova in the Amphiura 
was traced by opening several specimens. Eggs were found in all con- 
ditions of cleavage and larval growth, from very young specimens to an 
adult Crustacean (Copepod?). They are parasitic in nature, and pass 
their early life in the body of the Ophiuran. In several instances 
the ova of Amphiura were found with the ova of the parasite, but in 
most cases an amorphous reddish mass indicated the possible position of 
the ovarian gland of the host. The ova of the Ophiuran and those of 
the parasitic Crustacean can be readily distinguished by a, difference in 
color which is well marked. The eggs of the Crustacean are found in 
bright red or pink clusters. Those of the Amphiura are red and orange, 
and not in free packets. 
The ova of the Crustacean are unattached to the parent. They are 
often found without parent Crustacean. The development of the parasite 
will be treated in a special paper. None of the many attempts to produce 
artificially two Amphiurans by cutting the disk in halves led to positive 
success, although a six-armed Amphiuran cut in such a way that three 
arms were left in each half of the body lived as two individuals for a 
considerable time. They were not observed, however, to bud off new 
arms, as it was hoped they would do. Embryos with four and six arms 
were repeatedly found. Adults with six arms were common. 
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