WENRICH: SPERMATOGENESIS OF PHRYNOTETTIX MAGNUS. 83 
size can frequently be noticed throughout the pachytene stages. 
Examples of this may be seen at J, e, 7, 1, and o in figure 98 (Plate 8), 
which are drawn from the same individual as figure 58. Similar 
conditions are also to be found in other individuals, as will be seen in 
figure 97, a, c, e, j, and lL. 
This pair of chromosomes can be recognized in the spermatogonia 
by the presence, in the telophase, of the three most prominent granules, 
those I have numbered 1, 4, and 5 in the pachytene stages. Examples 
of such telophases are represented in figures 87-96. In two cases, 
where the chromosomes had become considerably elongated in the 
general diffusion process of the telophase, I was able to make out 
granules 2 and 3 also with their characteristic relative positions and 
sizes. These are shown in figures 95 and 96. Where both chromo- 
somes of the pair are recognizable in the same nucleus, there seems to 
be in every case a difference in size between the two middle granules 
(no. 4). This difference is probably directly related to the difference 
noted in the zygotene stage (fig. 58) and the pachytene stages (figs. 97 
and 98). 
Thus, aside from finding a striking degree of correspondence in the 
minute organization of the chromosome-pair B-for all the individuals 
studied (in the pachytene stages), it has also been possible to trace the 
pair through all the stages from the spermatogonia to the spermatid, 
except in the preleptotene and leptotene stages. Figures 30 (Plate 3) 
and 58 (Plate 5) show that conjugation is completed at a relatively 
early stage in the zygotene. This precocious conjugation is possibly 
facilitated by the relatively small size of this pair. The failure to 
recognize the pair in the leptotene and immediately preceding stages 
is probably due to the fact that it has not so great a differential stain- 
ing capacity as has pair A, and to the lack of sufficiently long con- 
tinued study with this object in view. 
A further peculiarity of chromosome-pair B may be seen upon an 
examination of figures 99 and 100 (Plate 9). There it will be seen 
that one end of the tetrad has a peculiar roughened or brush-like 
appearance, to which McClung (’14) has already called attention. 
It will be noticed in the same drawings that the accessory chromosome 
also presents a similar appearance. Furthermore, a like condition 
is to be seen at the longer end of C, as shown in figure 100, and at the 
end of some of the other autosomes, as seen in figure 99. The rough- 
ened contour of the accessory in both metaphase and anaphase of 
the first spermatocyte division was noted for Phrynotettix by Miss 
Pinney (’08), and has been described for other species of Orthoptera, 
Pee 
