84 BULLETIN: MUSEUM OF COMPARATIVE ZOOLOGY. 
for example by Davis (’08) for Dissosteira and Stenobothrus, and 
McClung (’14) for various Acrididae. But no one, so far as I am 
aware, has described such a condition for any of the autosomes. 
Figure 99 is from a slide that had been treated with Heidenhain’s 
iron-haematoxylin stain, but the destaining process had been carried 
farther than in most of the other slides. Figure 100 is from another 
individual, the slides of which had been stained by Flemming’s tri- 
color method, but had not been excessively differentiated. It will be 
noted that the autosomes in this figure do not exhibit the roughened 
synaptic ends that are seen in figure 99. It seems probable, therefore, 
that differences in the staining process may have much to do with 
the appearance or non-appearance of the roughened condition. In 
heavily stained slides even the accessory, as well as the tetrads B and 
C, may appear with a smooth contour. In this connection, I may call 
attention to these several points: — (1) Tetrad B is unequal in both 
the cases figured and the roughened end corresponds to the large 
distal granule on the larger conjugant (see fig. 64, Plate 6). (2) 
Tetrad C is likewise unequal and the roughened end also corresponds 
with the large distal granule at the end of the larger of the two com- 
ponents (see fig. 65). (3) The polar granules usually occur at the 
proximal end, 7. e. the end to which the spindle-fibers attach, and 
therefore the roughened tips of the autosomes in figure 99 probably 
correspond to the polar granules of these elements. (4) The accessory 
chromosome and the polar granules have the common property of 
remaining condensed while the rest of the chromatin is diffuse, as well 
as the common property exhibited in these two figures (99 and 100, 
Plate 9). The suggestion therefore offers itself that there may be 
some common physical or chemical properties underlying the corre- 
spondence in behavior between the accessory and the polar granules. 
3. Chromosome-pair C.— The drawings of chromosome-pair B in 
figure 64 (Plate 6) and those of C in figure 65 were made from sections 
cut from the same testis. An examination of the spermatogonial 
telophases of this individual revealed the larger members of each of 
these pairs very well defined, as indicated in figures 101-105 (Plate 9). 
No attempt was made to recognize the smaller members of these pairs, 
because they lacked characteristics, other than size, distinctive enough 
to make recognition certain. With the larger members of these pairs, 
however, the distinguishing features are so pronounced that I think 
there can be no doubt about the identification. 
I did not attempt to follow these elements through the preleptotene 
and leptotene stages, but I have no doubt that careful enough study 
