WENRICH: SPERMATOGENESIS OF PHRYNOTETTIX MAGNUS. 91 
especially true of the distal pole, as shown in figure 18. It would seem, 
therefore, that the vesicular membranes first became formed, then 
largely disappeared, and later reappeared in part. I am inclined to 
believe that they actually persist to a greater extent than is apparent. 
There cannot be any doubt, however, that the vesicles do coalesce at 
the polar end of the nucleus, for there the individual polar granules 
frequently fuse to form composite granules, such as may be seen, in 
figure 12 (Plate 1) and figures 14, 15, 16, and 19 (Plate 2). 
The first indication of the longitudinal split which forecasts the next 
mitosis was discernible at a stage such as is shown in figure 17. From 
this stage on to the metaphase, however, the split was clearly visible. 
I believe that the evidence here presented furnishes very good 
grounds for believing that the chromosomes do not lose their individ- 
uality in passing through the so-called ‘rest-stage’ between the two 
successive cell-divisions. 
d. The somatic Nuclei. 
Only slight attention has been given to somatic cells in connection 
with the subject of the individuality of chromosomes, but some points 
were noted which it seems worth while to record. The connective- 
tissue nuclei within the follicle always divide by the indirect or mi- 
totic method. The details are similar to those just described for the 
spermatogonia, except that individual chromosome-vesicles, even for 
the accessory, are less conspicuous — in fact, in my limited study of 
these cells I have not recognized the accessory chromosome with 
certainty. The only evidence of amitosis is a lobulated condition of 
the resting nuclei; that condition is a very characteristic one, but has 
no more significance as to amitosis than the lobulated appearance of 
the spermatogonial nuclei. In the diffused chromatin-stages — telo- 
phase, rest-stage, and early prophases — the polar granules appear, 
coalesce more or less to form composite granules, and separate out 
again just as they do in the spermatogonia. Furthermore, it is possi- 
ble to find chromosomes in the telophases that exhibit all the chief 
characteristics of the “selected”? chromosomes. For example, in 
Plate 9, B, figures 108, 109 and 110, are to be seen diffusing chromo- 
somes with the characteristic features of one of the larger members 
of chromosome-pair B. It would seem from this evidence that the 
same morphological constitution of individual chromosomes persisted 
even in these somatic cells. 
