164 BULLETIN: MUSEUM OF COMPARATIVE ZOOLOGY. 
authors have claimed them to be. For instance, one slide stained by 
this method showed the following distribution of red and green: in 
young odécytes every part—cytoplasm, nucleus and nucleolus—green; 
in larger odcytes, cytoplasm green, reticulum of nucleus brownish or 
maroon, nucleolus blue; in cells of the polyp, nucleus and nucleolus 
green, cytoplasm red; in the cells of embryos, chromosomes in a 
mitotic figure green, spindle fibres and asters red, cytoplasm maroon. 
- Sometimes the cytoplasm and nucleus of an embryo cell were of 
nearly identical colors, while in other cells of the same embryo they 
were of different colors. Hence in some cases the red and green stains 
would give the same results, in regard to chromatin and non-chromatin, 
as those produced by other stains; while in other cases, the relation 
of the red and green colors would lead to results different, and indeed 
opposite, from those obtained by other stains. It is almost certain, 
I think, that the green in these mixtures is not a specific chromatin 
stain, but is modified in its action by several factors, probably physical 
as well as chemical ones, as has been maintained by Heine (’95-6), 
Mathews. (’98), Fischer (’99), and Tellyesniezky (:05). 
III. Observations. 
A. PENNARIA TIARELLA. 
1. Growing eggs.— In the present investigation no attempt has 
been made to work out the odgenesis, except to a slight extent in the 
matter of the relation of the chromatin to the nucleolus. Smallwood 
(99) and C. W. Hargitt (:00, :04°) have paid some attention to oogen- 
esis and growth phenomena; what I have seen is, in the main, con- 
firmatory of their results. In addition to the absorption of oécytes 
by the growing egg, I may mention another possible method of nourish- 
ment not considered by my predecessors. In the later stages of growth, 
when nearly all the odcytes have been absorbed, the large eggs seemed 
generally to have several short pseudopodia attached to the entoderm 
of the manubrium. ' This suggests that during this period of growth 
nourishment may be obtained directly by absorption from the manu- 
brium, much as in the case of Eudendrium (C. W. Hargitt, :04°, 
Congdon, :06) and Clava (Harm, :03, Hargitt, :06). 
In odcytes before the growth period the chromatin of the nucleus is 
rather deeply stainable, and is arranged in masses near the periphery 
of the nucleus. These chromatin masses occupy a delicate linin 
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