Harairr: Pennaria tiarella and Tubularia crocea. 
Fig. 
Fia. 
Fig. 
Fig. 
Fig. 
Fig. 
25a. 
25b. 
26. 
27. 
28. 
29. 
PLATE 4. 
Figs. 25-28 Pennaria tiarella. Fig. 29 Tubularia crocea. 
Germ nuclei of unequal size mutually flattened. Bouin’s fluid, 
iron hematoxylin and Congo red. XX 1600. 
Another section of same nuclei, showing that the concentration of 
the chromatin begins on one side of the nuclei. X 2250. 
First-cleavage spindle forming. Germ nuclei have not fused; the 
chromatin strands, in two groups, have not yet formed definitive 
chromosomes. Bouin’s fluid, iron hematoxylin and Congo red. 
x 1600. 
First-cleavage spindle forming. Germ nuclei have already fused 
into a single vesicle; chromosomes not yet formed. Bouin’s 
fluid, picro-hematoxylin. > 1600. 
Second-cleavage spindle almost completed, nuclear membrane not 
yet ruptured. Chromosomes forming. Flemming’s fluid, iron 
hematoxylin. X 1600. 
Group of germ cells from gonophore; 0’go'., odgonia before last 
division; o’go”., odgonia in last division; o’cy'., odcyte formed, 
chromatin in dense mass; o’cy., chromatin of odcyte beginning 
to form a thread, nucleolus present; o’cy*., odcyte after growth © 
has started, chromatin ina spireme. Corrosive-acetic, iron hema- 
toxylin. X 1600. 
