TRANSACTIONS OF SECTION 1, 657 
Those protamines or proteins which contain no lysin, namely, protamines of 
the salmin group, also zein, hordein, &c., give no evidence, so far as has been 
investigated, of nitrogen titratable by formol. The presence of such lysin (in 
the protein molecule) can be recognised by the development of N in the Van 
Slyke procedure and by the titration with formol. These last-named inyesti- 
gations have been carried out with the assistance of N. Gawrilow. 
7. The Metabolism of Arginin. Effects on the Formation of Creatin 
and Creatinin. By Professor W. H. Tuompson, M.D. 
Arginin, prepared from herring sperma by the method of Kossel, was adminis- 
tered to dogs, both by feeding and by hypodermic injection, in doses of two 
grammes of the carbonate, neutralised by adding hydrochloric acid. ; 
The output in the urine, of total nitrogen, urea nitrogen, ammonia nitrogen, 
and, in two cases, of amino-acid nitrogen was estimated. The results in these 
respects confirmed previous observations published by the author.* 
In addition, the excretion of preformed and total creatinin, 7.e., creatinin 
and creatin, was also determined by the Folin method. 
The experiments fall into two series : Series A, in which arginin chloride was 
given alone; series B, in which it was combined with an equimolecular amount 
of methyl citrate. The effects of methyl citrate administered without arginin 
were also observed. ‘The results are given in the following tables, the figures 
indicating an increase or decrease per cent. (decrease expressed by minus sign) 
above those of a preliminary average period :— 
I. Hffect on Preformed Creatinin. 
Observation. Feeding. Injection. Day After. 
Series A, AraGrintin ALONE, | 
| Per cent. Per cent, Per cent, / 
1 1:0 15°2 10 
2 — 35 20°6 3°6 
3 6°90 19:0 9°1 | 
Series B, ARGININ AND METHYL CITRATE. 
4 — 9:3 6°5 0:7 
5 — 48 11°0 — 
II. Effect on the Total Creatinin (i.e., Creatinin and Creatin). 
Serres A, Araintn ALONE. 
Per cent. Per cent. Per cent 1 
1 6°83 30°2 10°3 
2 — 53 21°3 — 15 
3 14:0 29°0 12°4 
Serres B, Ararntin AND Meruyt CITRATE. 
+ — 30 63°38 + 45 
5 Nil tig — 
Arginin therefore when injected causes an increase in the excretion of 
creatinin in the urine and also an excretion of creatin. 
It was found that when methyl citrate alone was given there was, in one case 
* Journal of Physiology, vol. xxxii., p. 1387; Ibid. Proc. Physiol. Soc., 
‘p. xxii.; also vol, xxxiii., p. 106. 
1912. 
UU 
