122 BOTANICAL GAZETTE [AUGUST | 
any evidence of a tryptic enzyme be obtained. Further studies have 
been made of this seed enzyme in the hope of throwing light on the 
processes occurring during germination. - 
FURTHER EXPERIMENTS WITH THE GERMINATING SEEDS OF PHASEOLUS © 
VULGARIS 
The following experiment was carried out to demonstrate the 
action of the seed ereptase of the bean on the proteoses to be optaine® 
from phaseolin, the principal globulin of the seed. Phaseolin was 
prepared according to OsBorNE’s? method from white medium field .| 
beans, the yield from two kilograms of beans amounting to something 4 
over 75°". A small amount of phaselin was also obt-ined by the 
alcoholic precipitation of a part of the solution from which the phaseo- | 
lin had separated on dialysis. This albumin gives a marked Adam 
kiewicz reaction. © mA 
‘ain an ereptase solution finely ground beans pele di 
nzyme solution + 10° phaseolin proteose solution + 9 drops of # 
. I, using boiled enzyme solution. oe 
tion in 25°¢ of water. The test 
zyme 30 ution +ro%¢ Witte peptone solution+9 drops of u 
asno.§3, using boiled enzyme solution. 2 
7th Annual Report of the Conn. Agric. Experin ee 
